Blar i NILU Brage på forfatter "Rundén-Pran, Elise"
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Advanced Respiratory Models for Hazard Assessment of Nanomaterials. Performance of Mono-, Co- and Tricultures
Camassa, Laura Maria Azzurra; Elje, Elisabeth; Mariussen, Espen; Longhin, Eleonora Marta; Dusinska, Maria; Zienolddiny-Narui, Shanbeh; Rundén-Pran, Elise (Peer reviewed; Journal article, 2022)Advanced in vitro models are needed to support next-generation risk assessment (NGRA), moving from hazard assessment based mainly on animal studies to the application of new alternative methods (NAMs). Advanced models must ... -
The alamar blue assay in the context of safety testing of nanomaterials
Longhin, Eleonora Marta; El Yamani, Naouale; Rundén-Pran, Elise; Dusinska, Maria (Peer reviewed; Journal article, 2022)The Alamar Blue (AB) assay is widely used to investigate cytotoxicity, cell proliferation and cellular metabolic activity within different fields of toxicology. The use of the assay with nanomaterials (NMs) entails specific ... -
The colony forming efficiency assay for toxicity testing of nanomaterials – Modifications for higher-throughput
Rundén-Pran, Elise; Mariussen, Espen; El Yamani, Naouale; Elje, Elisabeth; Longhin, Eleonora Marta (Peer reviewed; Journal article, 2022)To cope with the high number of nanomaterials manufactured, it is essential to develop high-throughput methods for in vitro toxicity screening. At the same time, the issue with interference of the nanomaterial (NM) with ... -
Current status and future challenges of genotoxicity OECD Test Guidelines for nanomaterials: a workshop report
Doak, Shareen H.; Andreoli, Cristina; Burgum, Michael J.; Chaudhry, Qasim; Bleeker, Eric; Bossa, Cecilia; Domenech, Josefa; Drobne, Damjana; Fessard, Valérie; Jeliazkova, Nina; Longhin, Eleonora Marta; Rundén-Pran, Elise; Stepnik, Maciej; El Yamani, Naouale; Catalán, Julia; Dusinska, Maria (Peer reviewed; Journal article, 2023)Genotoxicity testing for nanomaterials remains challenging as standard testing approaches require some adaptation, and further development of nano-specific OECD Test Guidelines (TGs) and Guidance Documents (GDs) are needed. ... -
Decitabine potentiates efficacy of doxorubicin in a preclinical trastuzumab-resistant HER2-positive breast cancer models
Buociková, Verona; Longhin, Eleonora Marta; Pilalis, Eleftherios; Mastrokalou, Chara; Miklíková, Svetlana; Cihova, Marina; Poturnayova, Alexandra; Mackova, Katarina; Bábelová, Andrea; Trnkova, Lenka; El Yamani, Naouale; Zheng, Congying; Rios-Mondragon, Ivan; Labudova, Martina; Csaderova, Lucia; Kuracinova, Kristina Mikus; Makovicky, Peter; Kučerová, Lucia; Matuskova, Miroslava; Cimpan, Mihaela-Roxana; Dusinska, Maria; Babal, Pavel; Chatziioannou, Aristotelis; Gábelová, Alena; Rundén-Pran, Elise; Smolkova, Bozena (Peer reviewed; Journal article, 2022)Acquired drug resistance and metastasis in breast cancer (BC) are coupled with epigenetic deregulation of gene expression. Epigenetic drugs, aiming to reverse these aberrant transcriptional patterns and sensitize cancer ... -
Decitabine-induced DNA methylation-mediated transcriptomic reprogramming in human breast cancer cell lines; the impact of DCK overexpression
Buociková, Verona; Tyciakova, Sylvia; Pilalis, Eleftherios; Mastrokalou, Chara; Urbanova, Maria; Matuskova, Miroslava; Demkova, Lucia; Medova, Veronika; Longhin, Eleonora Marta; Rundén-Pran, Elise; Dusinska, Maria; Rios Mondragon, Ivan; Cimpan, Mihaela-Roxana; Gábelová, Alena; Soltysova, Andrea; Smolkova, Bozena; Chatziioannou, Aristotelis (Peer reviewed; Journal article, 2022)Decitabine (DAC), a DNA methyltransferase (DNMT) inhibitor, is tested in combination with conventional anticancer drugs as a treatment option for various solid tumors. Although epigenome modulation provides a promising ... -
Different Sensitivity of Advanced Bronchial and Alveolar Mono- and Coculture Models for Hazard Assessment of Nanomaterials
Elje, Elisabeth; Mariussen, Espen; McFadden, Erin; Dusinska, Maria; Rundén-Pran, Elise (Peer reviewed; Journal article, 2023)For the next-generation risk assessment (NGRA) of chemicals and nanomaterials, new approach methodologies (NAMs) are needed for hazard assessment in compliance with the 3R’s to reduce, replace and refine animal experiments. ... -
Genotoxic effects of occupational exposure to glass fibres - A human biomonitoring study
Ceppi, Marcello; Smolkova, Bozena; Staruchova, Marta; Kazimirova, Alena; Barancokova, Magdalena; Volkovova, Katarina; Collins, Andrew Richard; Kocan, Anton; Dzupinkova, Zuzana; Horska, Alexandra; Buocikova, Verona; Tulinska, Jana; Liskova, Aurelia; Mikusova, Miroslava Lehotska; Wsolova, Ladislava; Kuba, Daniel; Rundén-Pran, Elise; El Yamani, Naouale; Longhin, Eleonora Marta; Halasova, Erika; Kyrtopoulos, Soterios; Bonassi, Stefano; Dusinska, Maria (Peer reviewed; Journal article, 2023)As part of a large human biomonitoring study, we conducted occupational monitoring in a glass fibre factory in Slovakia. Shopfloor workers (n = 80), with a matched group of administrators in the same factory (n = 36), were ... -
Genotoxicity of Nanomaterials: Advanced In Vitro Models and High Throughput Methods for Human Hazard Assessment - A Review
Kohl, Yvonne; Rundén-Pran, Elise; Mariussen, Espen; Hesler, Michelle; El Yamani, Naouale; Longhin, Eleonora Marte; Dusinska, Maria (Peer reviewed; Journal article, 2020)Changes in the genetic material can lead to serious human health defects, as mutations in somatic cells may cause cancer and can contribute to other chronic diseases. Genotoxic events can appear at both the DNA, chromosomal ... -
Hazard assessment of nanomaterials using in vitro toxicity assays: Guidance on potential assay interferences and mitigating actions to avoid biased results
El Yamani, Naouale; Rundén-Pran, Elise; Varet, Julia; Beus, Maja; Dusinska, Maria; Fessard, Valerie; Moschini, Elisa; Serchi, Tommaso; Cimpan, Mihaela-Roxana; Lynch, Iseult; Vinković Vrček, ivana (Peer reviewed; Journal article, 2024)The movement towards an animal-free testing approach for risk assessment represents a key paradigm shift in toxicology. Risk assessment of engineered and anthropogenic nanoscale materials (NM) is dependent on reliable ... -
Hazard identification of nanomaterials: In silico unraveling of descriptors for cytotoxicity and genotoxicity
El Yamani, Naouale; Mariussen, Espen; Gromelski, Maciej; Wyrzykowska, Ewelina; Grabarek, Dawid; Puzyn, Tomasz; Tanasescu, Speranta; Dusinska, Maria; Rundén-Pran, Elise (Peer reviewed; Journal article, 2022)Hazard identification and safety assessment of the huge variety of nanomaterials (NMs), calls for robust and validated toxicity screening tests in combination with cheminformatics approaches to identify factors that can ... -
Hepato(Geno)Toxicity Assessment of Nanoparticles in a HepG2 Liver Spheroid Model
Elje, Elisabeth; Mariussen, Espen; Moriones, Oscar H.; Bastus, Neus G.; Puntes, Victor; Kohl, Yvonne; Dusinska, Maria; Rundén-Pran, Elise (Peer reviewed; Journal article, 2020)(1) In compliance with the 3Rs policy to reduce, refine and replace animal experiments, the development of advanced in vitro models is needed for nanotoxicity assessment. Cells cultivated in 3D resemble organ structures ... -
High throughput toxicity screening and intracellular detection of nanomaterials
Collins, Andrew Richard; Balasubramanyam, Annangi; Rubio, Laura; Marcos, Ricard; Dorn, Marco; Merker, Carolin; Estrela-Lopis, Irina; Cimpan, Mihaela Roxana; Ibrahim, Mohamed; Cimpan, Emil; Ostermann, Melanie; Sauter, Alexander; El Yamani, Naouale; Shaposhnikov, Sergey; Chevillard, Sylvie; Paget, Vincent; Grall, Romain; Delic, Jozo; Goñi- de-Cerio, Felipe; Suarez-Merino, Blanca; Fessard, Valérie; Hogeveen, Kevin N; Fjellsbø, Lise Marie; Rundén-Pran, Elise; Brzicova, Tana; Topinka, Jan; João Silva, Maria; Leite, PE; Ribeiro, AR; Granjeiro, JM; Grafström, Roland; Prina-Mello, Adriele; Dusinska, Maria (Journal article; Peer reviewed, 2017) -
Immunotoxicity, genotoxicity and epigenetic toxicity of nanomaterials: New strategies for toxicity testing?
Dusinska, Maria; Tulinska, Jana; El Yamani, Naouale; Kuricova, Miroslava; Liskova, Aurelia; Rollerova, Eva; Rundén-Pran, Elise; Smolkova, Bozena (Journal article; Peer reviewed, 2017) -
In vitro genotoxicity testing of four reference metal nanomaterials, titanium dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
El Yamani, Naouale; Collins, Andrew Richard; Rundén-Pran, Elise; Fjellsbø, Lise Marie; Shaposhnikov, Sergey; Zienolddiny, Shanbeh; Dusinska, Maria (Journal article; Peer reviewed, 2016) -
Lack of mutagenicity of TiO2 nanoparticles in vitro despite cellular and nuclear uptake
El Yamani, Naouale; Rubio, Laura; García-Rodríguez, Alba; Kažimírová, Alena; Rundén-Pran, Elise; Barančoková, Magdaléna; Marcos, Ricard; Dusinska, Maria (Peer reviewed; Journal article, 2022)The potential genotoxicity of titanium dioxide (TiO2) nanoparticles (NPs) is a conflictive topic because both positive and negative findings have been reported. To add clarity, we have carried out a study with two cell ... -
Microfluidic In Vitro Platform for (Nano)Safety and (Nano)Drug Efficiency Screening
Kohl, Yvonne; Biehl, Margit; Spring, Sarah; Hesler, Michelle; Ogourtsov, Vladimir; Todorovic, Miomir; Owen, Joshua; Elje, Elisabeth; Kopecka, Kristina; Moriones, Oscar Hernando; Bastus, Neus G.; Simon, Peter; Dubaj, Tibor; Rundén-Pran, Elise; Puntes, Victor; William, Nicola; von Briesen, Hagen; Wagner, Sylvia; Kapur, Nikil; Mariussen, Espen; Nelson, Andrew; Gabelova, A; Dusinska, Maria; Velten, Thomas; Knoll, Thorsten (Peer reviewed; Journal article, 2021)Microfluidic technology is a valuable tool for realizing more in vitro models capturing cellular and organ level responses for rapid and animal‐free risk assessment of new chemicals and drugs. Microfluidic cell‐based devices ... -
The miniaturized enzyme-modified comet assay for genotoxicity testing of nanomaterials
El Yamani, Naouale; Rundén-Pran, Elise; Collins, Andrew Richard; Longhin, Eleonora Marta; Elje, Elisabeth; Hoet, Peter; Vrček, Ivana Vinković; Doak, Shareen H.; Fessard, Valérie; Dusinska, Maria (Peer reviewed; Journal article, 2022)The in vitro comet assay is a widely applied method for investigating genotoxicity of chemicals including engineered nanomaterials (NMs). A big challenge in hazard assessment of NMs is possible interference between the NMs ... -
NanoSolveIT Project: Driving nanoinformatics research to develop innovative and integrated tools for in silico nanosafety assessment
Afantitis, Antreas; Melagraki, Georgia; Isigonis, Panagiotis; Tsoumanis, Andreas; Varsou, Dimitra Danai; Valsami-Jones, Eugenia; Papadiamantis, Anastasios; Ellis, Laura-Jayne; Sarimveis, Haralambos; Doganis, Philip; Karatzas, Pantelis; Tsiros, Periklis; Liampa, Irene; Lobaskin, Vladimir; Greco, Dario; Serra, Angela; Kinaret, Pia Anneli Sofia; Saarimaki, Laura Aliisa; Grafström, Roland; Kohonen, Pekka; Nymark, Penny; Willighagen, Egon; Puzyn, Tomasz; Rybinska-Fryca, Anna; Lyubartsev, Alexander; Jensen, Keld Alstrup; Brandenburg, Gerit; Lofts, Stephen; Svendsen, Claus; Harrison, Samuel; Maier, Dieter; Tamm, Kaido; Janes, Jaak; Sikk, Lauri; Dusinska, Maria; Longhin, Eleonora Marte; Rundén-Pran, Elise; Mariussen, Espen; El Yamani, Naouale; Unger, Wolfgang; Radnik, Jörg; Tropsha, Alexander; Cohen, Yoram; Leszcynski, Jerzy; Hendren, Christine Ogilvie; Wiesner, Mark; Winkler, David; Suzuki, Noriyuki; Yoon, Tae Hyun; Choi, Jang-Sik; Gulumian, Mary; Lynch, Iseult (Peer reviewed; Journal article, 2020)Nanotechnology has enabled the discovery of a multitude of novel materials exhibiting unique physicochemical (PChem) properties compared to their bulk analogues. These properties have led to a rapidly increasing range of ... -
NILU's Strategic Institute Initiatives (SIS) 2015.
Aas, Wenche; Breivik, Knut; Fiebig, Markus; Høiskar, Britt Ann Kåstad; Pacyna, Elisabeth G; Rundén-Pran, Elise; Schlabach, Martin; Sundseth, Kyrre (NILU report;3/2016, Research report, 2016)Each of NILU’s Strategic Institute Initiatives(SIS) is required to deliver a popular science report annually to the Research Council. This report contains all of NILU’s annual SIS-reports for 2015.